Toksisitetstester

Marine algae:

Marine algae Skeletonema costatum (ISO 10253:2006 E).  In this test the organism is exposed to a range of test concentrations, and the inhibition of growth is recorded over a period of 72 hours.  The result is given as EC50, i.e. the concentration of the test substance that gives 50 % growth inhibition.

Freshwater algae:

Freshwater algae Pseudochirchneriella subcapitata  (ISO 8692:2004 E). In this test the organism is exposed to a range of test concentrations, and the inhibition of growth is recorded over a period of 72 hours.  The result is given as EC50, i.e. the concentration of the test substance that gives 50 % growth inhibition.

Crustacean:

Marine copepod Acartia tonsa (ISO 14669, with recommendations given by OSPARCOM). In this test the organism is exposed to a range of test concentrations, and the lethality is recorded over a period of 48 hours.  The result is given as LC50, i.e. the concentration of the test substance that gives 50 % mortality (immobilisation).

Sediment reworker:

Marine amphipod Corophium volutator (Paris Commission Guideline, 2005. PARCOM Protocols on Methods for the Testing of Chemicals Used in the Offshore Oil Industry. Part A:  A sediment bioassay using an amphipod Corophium sp.).  In this test the organism is exposed to a range of test concentrations mixed into the sediment, and the lethality is recorded over a period of 10 days.  The result is given as LC50, i.e. the concentration of the test substance that gives 50 % mortality (immobilisation).

Fish:

Marine fish larvae Scophthalmus maximus (Paris Commission Guideline, 2005. PARCOM Protocols on Methods for the Testing of Chemicals Used in the Offshore Oil Industry. Part B: Protocol for a Fish Acute Toxicity Test).  The test is a limit test in which the concentration is the highest concentration of the test substance that resulted in mortality that was not significantly different from the blank using the most sensitive test species of the other taxonomic groups tested (NOEC)